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An organized view of the transcripome



Library:1661 (dbEST ID)

NCI_CGAP_Lu19

Library Description

Organism:Homo sapiens
Developmental stage:adult
Organ:lung
Tissue:squamous cell carcinoma, poorly differentiated (4 pooled tumors, including primary and metastatic)
Vector:pT7T3D-PacI
Vector type:phagemid (ampicillin resistant)
Lab host:DH10B (phage-resistant)

1st strand cDNA was prepared from pooled lung tumor tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went througb one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.

Source: Robert Strausberg, Ph.D.

Gene Content Analysis

6,257 ESTs from this library were grouped into 3,961 UniGene entries (putative genes) [UniGene build #217, 04-Dec-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs TPM   UniGene Entry
2 320 Hs.8102 Ribosomal protein S20, 5'UTR region.
2 320 Hs.79088 EST from clone 43449, 5' end.
2 320 Hs.78466 CDNA FLJ52462 complete cds, moderately similar to Mus musculus proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 (Psmd8), mRNA.
2 320 Hs.7753 Calumenin.
2 320 Hs.77274 RNA for urokinase-type plasminogen activator.
2 320 Hs.77060 Proteasome (prosome, macropain) subunit, beta type, 6 (PSMB6), mRNA.
2 320 Hs.76873 CDNA FLJ35130 fis, clone PLACE6008793, highly similar to Hyaluronidase-2 precursor (EC 3.2.1.35).
2 320 Hs.76753 Endoglin mRNA, 3' end.
2 320 Hs.76394 Enoyl Coenzyme A hydratase, short chain, 1, mitochondrial, mRNA (cDNA clone MGC:1513 IMAGE:2907059).
2 320 Hs.76364 Interferon gamma responsive transcript IRT-1.
2 320 Hs.76224 CDNA FLJ53670 complete cds, highly similar to EGF-containing fibulin-like extracellularmatrix protein 1 precursor.
2 320 Hs.76152 Uterine water channel=28 kda erythrocyte integral membrane protein homolog [human, uterus, mRNA, 1340 nt].
2 320 Hs.75864 GP36b glycoprotein.
2 320 Hs.75348 Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha), mRNA (cDNA clone MGC:4018 IMAGE:2961448).
2 320 Hs.75243 Homolog of Drosophila female sterile homeotic.
2 320 Hs.7517 CDNA FLJ76577 complete cds.
2 320 Hs.74471 CDNA FLJ53043 complete cds, highly similar to Gap junction alpha-1 protein.
2 320 Hs.74405 HS1 protein.
2 320 Hs.73797 CDNA FLJ56720 complete cds, highly similar to Guanine nucleotide-binding protein alpha-15 subunit.
2 320 Hs.73722 Apurinic/apyrimidinic endonuclease.
2 320 Hs.7367 Full length insert cDNA clone ZD78D08.
2 320 Hs.7200 (clone Zap2) mRNA fragment.
2 320 Hs.71941 CDNA FLJ52298 complete cds.
2 320 Hs.716658 CDNA FLJ36461 fis, clone THYMU2014901, highly similar to HOMEOBOX PROTEIN SIX1.
2 320 Hs.716489 CDNA FLJ56760 complete cds, highly similar to Immediate early response gene 5 protein.
2 320 Hs.716471 CDNA FLJ33669 fis, clone BRAMY2028740.
2 320 Hs.716439 CDNA FLJ60659 complete cds, weakly similar to N-acetylglucosamine-6-phosphatedeacetylase (EC 3.5.1.25).
2 320 Hs.716424 CDNA FLJ90569 fis, clone OVARC1001596, highly similar to Homo sapiens ADP-ribosylation factor-like 2 binding protein (ARL2BP), mRNA.
2 320 Hs.716405 Aryl sulfotransferase mRNA, intron 1.
2 320 Hs.716403 Homolog of Nedd5 (hNedd5).
2 320 Hs.716400 Rer1 protein.
2 320 Hs.716374 SMRP.
2 320 Hs.716373 ERPROT 213-21.
2 320 Hs.716371 UEV1As (UBE2V) mRNA, alternatively spliced.
2 320 Hs.7158 MRNA; cDNA DKFZp566H073 (from clone DKFZp566H073).
2 320 Hs.715556 DNA-PK interaction protein (KIP).
2 320 Hs.715540 Nrf2=NF-E2-like basic leucine zipper transcriptional activator [human, hemin-induced K562 cells, mRNA, 2304 nt].
2 320 Hs.715499 (TL27) mRNA from (PC3) cell line.
2 320 Hs.714955 Transcribed locus.
2 320 Hs.714892 Transcribed locus.
2 320 Hs.714791 Jun oncogene, mRNA (cDNA clone IMAGE:3947905).
2 320 Hs.714737 Transcription factor tbx3 (TBX3).
2 320 Hs.714700 CDNA FLJ55932 complete cds, highly similar to Homo sapiens WW domain containing adaptor with coiled-coil (WAC), transcript variant 2, mRNA.
2 320 Hs.714418 Napsin A aspartic peptidase, mRNA (cDNA clone MGC:22526 IMAGE:4693995).
2 320 Hs.714402 Transcribed locus.
2 320 Hs.714343 Clone IMAGE:943037, mRNA sequence.
2 320 Hs.714322 Clone 37, 5cM region surrounding hepatocyte nuclear factor-1a/MODY3 mRNA.
2 320 Hs.714302 Endobrevin.
2 320 Hs.714301 Transcribed locus.
2 320 Hs.713907 Transcribed locus.
2 320 Hs.713721 Transcribed locus, moderately similar to NP_033464.2 ribosomal protein L13a [Mus musculus].
2 320 Hs.713632 CDNA FLJ33305 fis, clone BNGH42003529, highly similar to E3 ubiquitin protein ligase TRAF7 (EC 6.3.2.-).
2 320 Hs.713327 Transcribed locus.
2 320 Hs.712666 MRNA; cDNA DKFZp686K2379 (from clone DKFZp686K2379).
2 320 Hs.712551 CDNA FLJ53369 complete cds, highly similar to Carboxypeptidase E precursor (EC 3.4.17.10).
2 320 Hs.711055 T-cluster binding protein.
2 320 Hs.710420 PREDICTED: Homo sapiens hypothetical protein LOC100133105 (LOC100133105), mRNA.
2 320 Hs.710184 Transcribed locus.
2 320 Hs.710041 Transcribed locus, strongly similar to NP_990681.1 B-cell translocation gene 1, anti-proliferative [Gallus gallus].
2 320 Hs.709775 MRNA; cDNA DKFZp686I10116 (from clone DKFZp686I10116).
2 320 Hs.709639 Zinc finger protein 775, mRNA (cDNA clone MGC:4815 IMAGE:3546283).
2 320 Hs.709632 MRNA; cDNA DKFZp761C1717 (from clone DKFZp761C1717).
2 320 Hs.709513 ADP-ribosylation factor-like protein 7 (ARL7).
2 320 Hs.709439 CDNA FLJ57618 complete cds, moderately similar to Rattus norvegicus macrophage expressed gene 1 (Mpeg1), mRNA.
2 320 Hs.709327 UTP18, small subunit (SSU) processome component, homolog (yeast), mRNA (cDNA clone MGC:39182 IMAGE:4861495).
2 320 Hs.709321 Interferon-inducible mRNA (cDNA 1-8).
2 320 Hs.709313 Beta 2- mu =beta 2-microglobulin [human, SK-MEL-33 cells, mRNA Mutant, 433 nt].
2 320 Hs.709235 Keratin 6 isoform K6f (KRT6F).
2 320 Hs.709231 Chromosome 17q12-21 mRNA, clone pOV-2.
2 320 Hs.709226 P cadherin.
2 320 Hs.709208 CDNA FLJ39295 fis, clone OCBBF2012714, highly similar to GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(T) BETA SUBUNIT 1.
2 320 Hs.709184 Amyloid protein homologue.
2 320 Hs.706793 Clone 334-2C mRNA sequence.
2 320 Hs.705873 Transcribed locus.
2 320 Hs.705480 Protein tyrosine phosphatase-like (proline instead of catalytic arginine), member b, mRNA (cDNA clone IMAGE:4398482).
2 320 Hs.7036 CDNA FLJ56394 complete cds, highly similar to N-acetylglucosamine kinase (EC 2.7.1.59).
2 320 Hs.703354 Transcribed locus, moderately similar to NP_071432.2 melanoma antigen family F, 1 [Homo sapiens].
2 320 Hs.703326 Transcribed locus, moderately similar to NP_536849.1 cytochrome c oxidase subunit III [Homo sapiens].
2 320 Hs.70312 COX VIIa-L mRNA for liver-specific cytochrome c oxidase (EC 1.9.3.1.).
2 320 Hs.700699 Transcribed locus.
2 320 Hs.699486 Transcribed locus, moderately similar to NP_001035397.1 F-box protein 8 [Danio rerio].
2 320 Hs.699306 High molecular weight B cell growth factor mRNA sequence.
2 320 Hs.697228 Transcribed locus, strongly similar to XP_529414.2 PREDICTED: hypothetical protein [Pan troglodytes].
2 320 Hs.696364 CDNA FLJ33263 fis, clone ASTRO2006732, highly similar to Protein Wnt-5a precursor.
2 320 Hs.69517 Lymphocyte antigen 6 complex, locus K (LY6K), mRNA.
2 320 Hs.67776 Family with sequence similarity 83, member H, mRNA (cDNA clone IMAGE:3138324).
2 320 Hs.671456 CDNA FLJ45493 fis, clone BRTHA2008598.
2 320 Hs.671259 Transcribed locus.
2 320 Hs.668093 Transcribed locus.
2 320 Hs.664500 HOXC8 protein, exon 1.
2 320 Hs.663133 Ski oncogene mRNA.
2 320 Hs.661081 Transcribed locus.
2 320 Hs.660599 Transcribed locus.
2 320 Hs.659828 Transcribed locus.
2 320 Hs.659022 Transcribed locus, moderately similar to NP_006464.1 TAF6-like RNA polymerase II [Homo sapiens].
2 320 Hs.658245 Inositol polyphosphate 4-phosphatase type II-alpha.
2 320 Hs.657347 Muscleblind-like 2 (Drosophila), mRNA (cDNA clone IMAGE:4157895).
2 320 Hs.657267 CDNA FLJ14344 fis, clone THYRO1001142.
2 320 Hs.656751 CDNA FLJ33343 fis, clone BRACE2003614.
2 320 Hs.656425 CDNA clone IMAGE:4932495.

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Source: NCBI UniGene

EST Sequences

6,901 EST sequences from this library have been submitted to the public sequence database as of 04-Dec-2008.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

  • lung
  • lung tumor
  • adult
  • normalized

Source: NCBI Clone Registry



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